Protein patterns for cultivar identification
نویسندگان
چکیده
Heras, J.M., M.L. Marina, and M.C. Garcia, Development of a Perfusion Ion-Exchange Chromatography Method for the Separation of Soybean Proteins and Its Application to Cultivar Identification, J. Chromatogr. A 1153:97–103, 2007. Fractionation of soybean proteins has been obtained using a developed method of perfusion ion-exchange chromatography. The method comprises a two-step gradient optimized for buffer concentration, buffer pH, and salt and for temperature. Aqueous solutions of samples were examined. Resolution of proteins in soybean isolates into 11 components could be achieved within 9 min. The protein pattern was observed in all isolates studied, but the quantity of each component (measured from peak area) allowed differentiation between soybeans. Multivariate methods were used for pattern recognition and the classification of samples.
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